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Article ; Online: Die Prävalenz von SARS-CoV-2-IgG-AK liegt bei 1,2% : Screening bei asymptomatischen ambulanten Patienten.

Herrmann, Burkhard L

2020 Volume 162, Issue 14, Page(s) 44–46

Abstract: Patients with newly diagnosed COVID-19 (coronavirus disease 2019) develop antibodies against SARS-CoV-2 (severe acute respiratory syndrome coronavirus 2). To date, few data have been obtained of the prevalence of SARS-CoV-2-antibodies in general ... ...

Title translation	The prevalence rate of anti-SARS-CoV-2-IgG is 1.2% - Screening in asymptomatic outpatients in Germany (Northrhine-Westfalia).
Abstract	Patients with newly diagnosed COVID-19 (coronavirus disease 2019) develop antibodies against SARS-CoV-2 (severe acute respiratory syndrome coronavirus 2). To date, few data have been obtained of the prevalence of SARS-CoV-2-antibodies in general population and in asymptomatic outpatients in Germany.From March 26 to June 4 2020, 415 asymptomatic outpatients were tested prospectively in Northrhine-Westfalia (Germany), to detect SARS-CoV-2-IgG-antibodies. In case of a positive result, anti-SARS-CoV-2-IgA was determined additionally.5 of 415 asymptomatic outpatients had positive SARS-CoV-2-IgG-antibodies with a calculated prevalence of 1.2%. Reference range of anti-SARS-CoV-2-IgA and IgG was defined as ratio for negative < 0.8, borderline 0.8-1.1 and > 1.1 positive. The mean concentration of SARS-CoV-2-IgG-antibodies of the positive 5 outpatients was lower than in symptomatic patients with COVID-19 (n = 12) and positive PCR of SARS-CoV-2 (3.04 ± 2.58 versus 8.05 ± 6.70; p = 0.002). 4 of 5 patients had elevated SARS-CoV-2-IgA-antibodies (1.61 ± 0.82). In 408 screening-outpatients with negative anti-SARS-CoV-2-ELISA-IgG (< 0.8), the mean ratio was 0.25 ± 0.13. Two patients were in the borderline range (0.83 and 0.86).The prevalence of 1.2% of SARS-CoV-2-IgG-antibodies and consequently the rate of infection in asymptomatic outpatients in Northrhine-Westfalia (Germany) is low. The impact of virus neutralisation by antibodies and consequently immunization is the challenge of further investigations.
MeSH term(s)	Antibodies, Viral/blood ; Asymptomatic Infections ; Betacoronavirus ; COVID-19 ; Coronavirus Infections/diagnosis ; Germany ; Humans ; Immunoglobulin A/blood ; Immunoglobulin G/blood ; Outpatients ; Pandemics ; Pneumonia, Viral/diagnosis ; Prevalence ; SARS-CoV-2
Chemical Substances	Antibodies, Viral ; Immunoglobulin A ; Immunoglobulin G
Keywords	covid19
Language	German
Publishing date	2020-08-11
Publishing country	Germany
Document type	Journal Article ; Review
ZDB-ID	1478211-x
ISSN	1613-3560 ; 1438-3276
ISSN (online)	1613-3560
ISSN	1438-3276
DOI	10.1007/s15006-020-0750-y
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Article ; Online: Die Prävalenz von SARS-CoV-2-IgG-AK liegt bei 1,2% ; Screening bei asymptomatischen ambulanten Patienten

Herrmann, Burkhard L.

MMW - Fortschritte der Medizin

2020 Volume 162, Issue 14, Page(s) 44–46

Keywords	General Medicine ; covid19
Language	German
Publisher	Springer Science and Business Media LLC
Publishing country	us
Document type	Article ; Online
ZDB-ID	1415147-9
ISSN	1438-3276
ISSN	1438-3276
DOI	10.1007/s15006-020-0750-y
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article: Die Prävalenz von SARS-CoV-2-IgG-AK liegt bei 1,2% : Screening bei asymptomatischen ambulanten Patienten./ [The prevalence rate of anti-SARS-CoV-2-IgG is 1.2% - Screening in asymptomatic outpatients in Germany (Northrhine-Westfalia)]

Herrmann, Burkhard L

MMW Fortschr Med

Abstract	Patients with newly diagnosed COVID-19 (coronavirus disease 2019) develop antibodies against SARS-CoV-2 (severe acute respiratory syndrome coronavirus 2). To date, few data have been obtained of the prevalence of SARS-CoV-2-antibodies in general population and in asymptomatic outpatients in Germany.From March 26 to June 4 2020, 415 asymptomatic outpatients were tested prospectively in Northrhine-Westfalia (Germany), to detect SARS-CoV-2-IgG-antibodies. In case of a positive result, anti-SARS-CoV-2-IgA was determined additionally.5 of 415 asymptomatic outpatients had positive SARS-CoV-2-IgG-antibodies with a calculated prevalence of 1.2%. Reference range of anti-SARS-CoV-2-IgA and IgG was defined as ratio for negative < 0.8, borderline 0.8-1.1 and > 1.1 positive. The mean concentration of SARS-CoV-2-IgG-antibodies of the positive 5 outpatients was lower than in symptomatic patients with COVID-19 (n = 12) and positive PCR of SARS-CoV-2 (3.04 ± 2.58 versus 8.05 ± 6.70; p = 0.002). 4 of 5 patients had elevated SARS-CoV-2-IgA-antibodies (1.61 ± 0.82). In 408 screening-outpatients with negative anti-SARS-CoV-2-ELISA-IgG (< 0.8), the mean ratio was 0.25 ± 0.13. Two patients were in the borderline range (0.83 and 0.86).The prevalence of 1.2% of SARS-CoV-2-IgG-antibodies and consequently the rate of infection in asymptomatic outpatients in Northrhine-Westfalia (Germany) is low. The impact of virus neutralisation by antibodies and consequently immunization is the challenge of further investigations.
Keywords	covid19
Publisher	WHO
Document type	Article
Note	WHO #Covidence: #710032
Database	COVID19

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Article: Quantitative measurement of IgG to SARS-CoV-2 antigens using monoclonal antibody-based enzyme-linked immunosorbent assays.

Sander, Ingrid / Kespohl, Sabine / Zahradnik, Eva / Göcke, Philipp / Hosbach, Ingolf / Herrmann, Burkhard L / Brüning, Thomas / Raulf, Monika

Clinical & translational immunology

2022 Volume 11, Issue 2, Page(s) e1369

Abstract: Objective: Standardised quantitative analysis of the humoral immune response to SARS-CoV-2 antigens may be useful for estimating the extent and duration of immunity. The aim was to develop enzyme-linked immunosorbent assays (ELISAs) for the ... ...

Abstract	Objective: Standardised quantitative analysis of the humoral immune response to SARS-CoV-2 antigens may be useful for estimating the extent and duration of immunity. The aim was to develop enzyme-linked immunosorbent assays (ELISAs) for the quantification of human IgG antibodies against SARS-CoV-2 antigens. Methods: Enzyme-linked immunosorbent assays were developed based on monoclonal antibodies against human IgG and recombinant SARS-CoV-2 antigens (Spike-S1 and Nucleocapsid). The WHO 67/086 immunoglobulin and WHO 20/136 SARS-CoV-2 references were used for standardisation. Sera of a study group of COVID-19-positive subjects ( Results: The ELISA results were repeatable and traceable to international units because of their parallelism to both WHO references. In the study group, median anti-S1-IgG concentrations were 102 BAU mL Conclusions: The quantitative ELISAs to measure IgG binding to SARS-CoV-2 antigens have good analytical and clinical performance characteristics and units traceable to international standards.
Language	English
Publishing date	2022-01-30
Publishing country	Australia
Document type	Journal Article
ZDB-ID	2694482-0
ISSN	2050-0068
ISSN	2050-0068
DOI	10.1002/cti2.1369
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Article: Auch der Mann braucht Östrogene. Die fatalen Folgen eines sehr seltenen Aromatase-Mangels

Herrmann, Burkhard L.

Essener Unikate

2005 Volume -, Issue 25, Page(s) 46

Language	German
Document type	Article
ZDB-ID	1166117-3
ISSN	0941-746X ; 0944-6060
Database	Current Contents Medicine

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Article ; Online: A methodological inter-comparison study on the detection of surface contaminant sodium dodecyl sulfate applying ambient- and vacuum-based techniques.

Giovannozzi, Andrea M / Hornemann, Andrea / Pollakowski-Herrmann, Beatrix / Green, Felicia M / Gunning, Paul / Salter, Tara L / Steven, Rory T / Bunch, Josephine / Portesi, Chiara / Tyler, Bonnie J / Beckhoff, Burkhard / Rossi, Andrea Mario

Analytical and bioanalytical chemistry

2018 Volume 411, Issue 1, Page(s) 217–229

Abstract: Biomedical devices are complex products requiring numerous assembly steps along the industrial process chain, which can carry the potential of surface contamination. Cleanliness has to be analytically assessed with respect to ensuring safety and efficacy. ...

Abstract	Biomedical devices are complex products requiring numerous assembly steps along the industrial process chain, which can carry the potential of surface contamination. Cleanliness has to be analytically assessed with respect to ensuring safety and efficacy. Although several analytical techniques are routinely employed for such evaluation, a reliable analysis chain that guarantees metrological traceability and quantification capability is desirable. This calls for analytical tools that are cascaded in a sensible way to immediately identify and localize possible contamination, both qualitatively and quantitatively. In this systematic inter-comparative approach, we produced and characterized sodium dodecyl sulfate (SDS) films mimicking contamination on inorganic and organic substrates, with potential use as reference materials for ambient techniques, i.e., ambient mass spectrometry (AMS), infrared and Raman spectroscopy, to reliably determine amounts of contamination. Non-invasive and complementary vibrational spectroscopy techniques offer a priori chemical identification with integrated chemical imaging tools to follow the contaminant distribution, even on devices with complex geometry. AMS also provides fingerprint outputs for a fast qualitative identification of surface contaminations to be used at the end of the traceability chain due to its ablative effect on the sample. To absolutely determine the mass of SDS, the vacuum-based reference-free technique X-ray fluorescence was employed for calibration. Convex hip liners were deliberately contaminated with SDS to emulate real biomedical devices with an industrially relevant substance. Implementation of the aforementioned analytical techniques is discussed with respect to combining multimodal technical setups to decrease uncertainties that may arise if a single technique approach is adopted. Graphical abstract ᅟ.
MeSH term(s)	Humans ; Reference Standards ; Sodium Dodecyl Sulfate/analysis ; Sodium Dodecyl Sulfate/standards ; Spectrum Analysis/methods ; Surface Properties ; Vacuum
Chemical Substances	Sodium Dodecyl Sulfate (368GB5141J)
Language	English
Publishing date	2018-11-06
Publishing country	Germany
Document type	Comparative Study ; Journal Article
ZDB-ID	201093-8
ISSN	1618-2650 ; 0016-1152 ; 0372-7920
ISSN (online)	1618-2650
ISSN	0016-1152 ; 0372-7920
DOI	10.1007/s00216-018-1431-x
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Article: A methodological inter-comparison study on the detection of surface contaminant sodium dodecyl sulfate applying ambient- and vacuum-based techniques

Giovannozzi, Andrea M / Andrea Hornemann / Beatrix Pollakowski-Herrmann / Felicia M. Green / Paul Gunning / Tara L. Salter / Rory T. Steven / Josephine Bunch / Chiara Portesi / Bonnie J. Tyler / Burkhard Beckhoff / Andrea Mario Rossi

Analytical and bioanalytical chemistry. 2019 Jan., v. 411, no. 1

2019

Abstract	Biomedical devices are complex products requiring numerous assembly steps along the industrial process chain, which can carry the potential of surface contamination. Cleanliness has to be analytically assessed with respect to ensuring safety and efficacy. Although several analytical techniques are routinely employed for such evaluation, a reliable analysis chain that guarantees metrological traceability and quantification capability is desirable. This calls for analytical tools that are cascaded in a sensible way to immediately identify and localize possible contamination, both qualitatively and quantitatively. In this systematic inter-comparative approach, we produced and characterized sodium dodecyl sulfate (SDS) films mimicking contamination on inorganic and organic substrates, with potential use as reference materials for ambient techniques, i.e., ambient mass spectrometry (AMS), infrared and Raman spectroscopy, to reliably determine amounts of contamination. Non-invasive and complementary vibrational spectroscopy techniques offer a priori chemical identification with integrated chemical imaging tools to follow the contaminant distribution, even on devices with complex geometry. AMS also provides fingerprint outputs for a fast qualitative identification of surface contaminations to be used at the end of the traceability chain due to its ablative effect on the sample. To absolutely determine the mass of SDS, the vacuum-based reference-free technique X-ray fluorescence was employed for calibration. Convex hip liners were deliberately contaminated with SDS to emulate real biomedical devices with an industrially relevant substance. Implementation of the aforementioned analytical techniques is discussed with respect to combining multimodal technical setups to decrease uncertainties that may arise if a single technique approach is adopted. Graphical abstract ᅟ
Keywords	Raman spectroscopy ; X-ray fluorescence spectroscopy ; fluorescence ; geometry ; image analysis ; mass spectrometry ; medical equipment ; sodium dodecyl sulfate ; traceability ; uncertainty
Language	English
Dates of publication	2019-01
Size	p. 217-229.
Publishing place	Springer Berlin Heidelberg
Document type	Article
ISSN	1618-2642
DOI	10.1007/s00216-018-1431-x
Database	NAL-Catalogue (AGRICOLA)

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Article ; Online: Variability in ratings of trustworthiness across the menstrual cycle.

Ball, Anna / Wolf, Claudia C / Ocklenburg, Sebastian / Herrmann, Burkhard L / Pinnow, Marlies / Brüne, Martin / Wolf, Oliver T / Güntürkün, Onur

Biological psychology

2013 Volume 93, Issue 1, Page(s) 52–57

Abstract: This study investigated how trusting behavior varies in naturally cycling women, as a function of sex and attractiveness of players in a trust game, at three distinct phases of the menstrual cycle. Women acted more cautiously in an investment game at the ...

Abstract	This study investigated how trusting behavior varies in naturally cycling women, as a function of sex and attractiveness of players in a trust game, at three distinct phases of the menstrual cycle. Women acted more cautiously in an investment game at the preovulatory phase, compared to the menstrual and the mid-luteal phase. Reduced willingness to trust in strangers was particularly expressed toward male players at this time. The increase of estradiol levels from menses to the preovulatory phase was negatively correlated with trust in attractive male other players, whereas the increase of progesterone levels from menses to the mid-luteal phase was positively associated with trust in unattractive female other players. No particular contribution of a single hormone level could be identified for the generally reduced willingness to trust in strangers in the preovulatory phase. Thus, the results emphasize the impact of the menstrual cycle on interpersonal trust, although the exact mode of hormonal action needs to be further investigated.
MeSH term(s)	Adult ; Estradiol/blood ; Female ; Humans ; Judgment ; Male ; Menstrual Cycle/psychology ; Progesterone/blood ; Trust
Chemical Substances	Progesterone (4G7DS2Q64Y) ; Estradiol (4TI98Z838E)
Language	English
Publishing date	2013-04
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	185105-6
ISSN	1873-6246 ; 0301-0511
ISSN (online)	1873-6246
ISSN	0301-0511
DOI	10.1016/j.biopsycho.2013.01.005
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Article ; Online: Kardiovaskuläre Effekte oraler Antidiabetika.

Herrmann, Burkhard L / Erbel, Raimund / Janssen, Onno E / Mann, Klaus

Herz

2004 Volume 29, Issue 5, Page(s) 510–518

Abstract: In the recent years there has been increasing interest in the effects of oral hypoglycemic drugs on the cardiovascular system. This has arisen because of recognitions that thiazolidine-diones, peroxisome proliferators-activated receptor gamma (PPAR-gamma) ...

Title translation	Cardiovascular effects of oral hypoglycemie drugs.
Abstract	In the recent years there has been increasing interest in the effects of oral hypoglycemic drugs on the cardiovascular system. This has arisen because of recognitions that thiazolidine-diones, peroxisome proliferators-activated receptor gamma (PPAR-gamma), may have antiatherogenic actions and that sulphonylureas are capable of closing the ATP-dependent potassium channel. PPAR-gamma agonists exert antiatherogenic action by inhibition the production of monocyte inflammatory cytokines, inhibition of expression of adhesion molecules in endothelial cells, inhibition of the proliferation of vascular smooth muscle cells and have antioxidative effects. The United Kingdom Prospective Diabetes Study (UKPDS), published in 1998, found that the use of sulphonylureas had no increase in cardiovascular mortality and that metformin therapy in obese individuals with type 2 diabetes mellitus was associated with reduced cardiovascular death. Recently, the STOP-NIDDM trial has been shown that patients with impaired glucose tolerance treated with the alpha-glucosidase inhibitor acarbose had a significant reduction in the risk of cardiovascular disease. Currently, the results of the UKPDS trial are the only available clinical data on which to base the choice of treatment for type 2 diabetic patients. When a glucose-lowering oral drug is considered necessary and is not contraindicated, the firstline choice is a sulphonylurea or a glinide (repaglinide or nateglinide) for diabetics who are not overweight and metformin for those who are.
MeSH term(s)	Administration, Oral ; Cardiovascular Diseases/epidemiology ; Cardiovascular Diseases/prevention & control ; Comorbidity ; Diabetes Mellitus, Type 2/drug therapy ; Diabetes Mellitus, Type 2/epidemiology ; Humans ; Hypoglycemic Agents/administration & dosage ; Incidence ; Risk Assessment ; Risk Factors
Chemical Substances	Hypoglycemic Agents
Language	German
Publishing date	2004-08
Publishing country	Germany
Document type	English Abstract ; Journal Article ; Review
ZDB-ID	8262-4
ISSN	1615-6692 ; 0340-9937 ; 0946-1299
ISSN (online)	1615-6692
ISSN	0340-9937 ; 0946-1299
DOI	10.1007/s00059-004-2563-7
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Article: Changes in calf muscle elasticity in hypogonadal males before and after testosterone substitution as monitored by magnetic resonance elastography.

Brauck, Katja / Galbán, Craig J / Maderwald, Stefan / Herrmann, Burkhard L / Ladd, Mark E

European journal of endocrinology

2007 Volume 156, Issue 6, Page(s) 673–678

Abstract: Purpose: We sought to determine whether differences in muscle elasticity between healthy age-related controls and patients with hypogonadism could be measured by a new, non-invasive method termed magnetic resonance elastography (MRE).: Materials and ... ...

Abstract	Purpose: We sought to determine whether differences in muscle elasticity between healthy age-related controls and patients with hypogonadism could be measured by a new, non-invasive method termed magnetic resonance elastography (MRE). Materials and methods: Twelve hypogonadal (21-68 years) and twenty-seven healthy age-related men (20-76 years) were examined. In the hypogonadism group, serum testosterone levels were compared and MRE was conducted prior to and after 6 months of therapy in 6 of the 12 patients. MRE was performed by mechanically exciting the soleus muscle that was used because of its uniformly distributed muscle fibers, size, and accessibility, with a custom designed piezoelectric-actuator using a modified phase-contrast sequence. For mechanical excitation the actuator lever was placed on the anterior surface of the calf. The subjects had to maintain a force of 0-20% of their maximum voluntary contraction against a home-built footplate that was mounted on the MR table. All images were phase unwrapped and reconstructed into shear modulus elastograms using the local frequency estimation technique. Results: Testosterone levels were significantly higher after 6 months of treatment. A statistical difference in the shear modulus was observed prior to and after 6 months of testosterone therapy and was nearly the same as in the healthy age-related control group. Conclusion: MRE seems to be a promising technique for the evaluation of therapeutic effects in patients with hypogonadism and possibly in other diseases with muscular effects.
MeSH term(s)	Adult ; Aged ; Androgens/administration & dosage ; Androgens/blood ; Drug Monitoring/instrumentation ; Drug Monitoring/methods ; Elasticity ; Humans ; Hypogonadism/drug therapy ; Hypogonadism/physiopathology ; Magnetic Resonance Imaging/methods ; Male ; Middle Aged ; Muscle, Skeletal/physiopathology ; Testosterone/administration & dosage ; Testosterone/blood
Chemical Substances	Androgens ; Testosterone (3XMK78S47O)
Language	English
Publishing date	2007-06
Publishing country	England
Document type	Controlled Clinical Trial ; Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	1183856-5
ISSN	1479-683X ; 0804-4643
ISSN (online)	1479-683X
ISSN	0804-4643
DOI	10.1530/EJE-06-0694
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